Insertion junction: LMJ.RY0402.129927_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.129927
Chromosome:chromosome 10
Location:13797
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g417500 CYN20E,CYN20-5,CYN9 (1 of 1) PF00160//PF04969 - Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD (Pro_isomerase) // CS domain (CS); Cyclophilin 20-5 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCCCTCAGACTGCAAGCAAGCTGGCATA
Internal bar code:GAAGGCGGAGGAACGCACGGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:739
LEAP-Seq percent confirming:99.6617
LEAP-Seq n confirming:2062
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:TAGTGGCACAGCAGATTTCG
Suggested primer 2:GACTCCAACAGCTTTCCTGC