| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.129931 |
| Chromosome: | chromosome 16 |
| Location: | 845258 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g647800 | (1 of 1) IPR000104//IPR001932//IPR015655 - Antifreeze protein, type I // PPM-type phosphatase domain // Protein phosphatase 2C | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCACCCCGCCCACCGCGCCGGCCATGCC |
| Internal bar code: | GTGGGGAGTTCGGTTCCTACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 17 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTATTAAACGCGTGCCTA |
| Suggested primer 2: | GCACCCACAGTACCACCTCT |