| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.129942 |
| Chromosome: | chromosome 11 |
| Location: | 3127587 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g479200 | (1 of 5) PF00995 - Sec1 family (Sec1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTTACGGTTTCATTGTAATTGATTTTAG |
| Internal bar code: | CTTCTACCGCGAGAGTTCGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 318 |
| LEAP-Seq percent confirming: | 89.0 |
| LEAP-Seq n confirming: | 445 |
| LEAP-Seq n nonconfirming: | 55 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACCTACACATGTGCCCAG |
| Suggested primer 2: | AACTGCCCCAAACTATGCAC |