| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.129947 |
| Chromosome: | chromosome 13 |
| Location: | 2880357 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g583450 | EZY17 | Early zygote expressed protein; (1 of 6) PTHR15907:SF21 - DUF614 FAMILY PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAGACGTATCTCTAGAAGCAGAGCCTGC |
| Internal bar code: | ATGTGTGACGCGTAGACGACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 840 |
| LEAP-Seq percent confirming: | 99.5897 |
| LEAP-Seq n confirming: | 971 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGCCCAACTAGCCAACCT |
| Suggested primer 2: | TCATGCGCTGTTCTTCAAAC |