| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.129968 |
| Chromosome: | chromosome 9 |
| Location: | 7244641 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g412550 | (1 of 3) IPR000104//IPR005069 - Antifreeze protein, type I // Nucleotide-diphospho-sugar transferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTATCTGTCAGCGGGGCCAAACCGGTGG |
| Internal bar code: | CGGCGACATCGGATTTCTTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 216 |
| LEAP-Seq percent confirming: | 99.4012 |
| LEAP-Seq n confirming: | 498 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGTTAAATCATGAAGCGG |
| Suggested primer 2: | CTGCTGTGTGCAGGACATCT |