Insertion junction: LMJ.RY0402.129984_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.129984
Chromosome:chromosome 17
Location:3055519
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g720700 ANK14 Predicted protein with ankyrin repeats; (1 of 7) PF12796//PF13637 - Ankyrin repeats (3 copies) (Ank_2) // Ankyrin repeats (many copies) (Ank_4) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTGCGTGTTGGGGTCGGCGCCGCCCTTG
Internal bar code:ACACTCTGATCCATTTGGTTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:528
LEAP-Seq percent confirming:99.8667
LEAP-Seq n confirming:2247
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GGGAGAGGAGCCAACATACA
Suggested primer 2:TGAGAAGTGACGGCGTAGTG