| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.130021 |
| Chromosome: | chromosome 4 |
| Location: | 2907871 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g224550 | GT90F37,GT90-37 | (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90); GT90 family protein 37 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTAGTTCGTTTCCCCTGGCGGGTGGTCAT |
| Internal bar code: | CCGTCGCATCGAGGTCGGGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 593 |
| LEAP-Seq percent confirming: | 99.7325 |
| LEAP-Seq n confirming: | 1864 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTACGGCACCATCAACAC |
| Suggested primer 2: | GGAGCTGTACGAAGTCAGCC |