| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.130037 |
| Chromosome: | chromosome 5 |
| Location: | 3332388 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g240550 | TLA1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGACATTTTCGGCGTTCTTTGCAGTCAGA |
| Internal bar code: | GCGGGGGCCCAAGGCCGTGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 827 |
| LEAP-Seq percent confirming: | 99.5546 |
| LEAP-Seq n confirming: | 1341 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTTGAGAGGAGGAGCC |
| Suggested primer 2: | CTGCAGTCCTCGATTCAACA |