Insertion junction: LMJ.RY0402.130043_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.130043
Chromosome:chromosome 12
Location:4090347
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g517850 (1 of 18) PF12906 - RING-variant domain (RINGv) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGTAGATGCAGCAGTGGCTCCCTGAGCTC
Internal bar code:ACCCTTCGATATGAGGGGGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:845
LEAP-Seq percent confirming:99.5418
LEAP-Seq n confirming:12818
LEAP-Seq n nonconfirming:59
LEAP-Seq n unique pos:57

Suggested primers for confirmation by PCR

Suggested primer 1:CTTCGTGGCGTTCATAGTCA
Suggested primer 2:GAGAGAGGCTGTCAGGTTGG