| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.130061 |
| Chromosome: | chromosome_10 |
| Location: | 941028 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g424600 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCGCCTCCATCCACCTGGCCAGTCAACCAT |
| Internal bar code: | CGCGTCTTCCGGCCTCGGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 55 |
| LEAP-Seq percent confirming: | 98.3969 |
| LEAP-Seq n confirming: | 5033 |
| LEAP-Seq n nonconfirming: | 82 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGGTTACGCTCATGATTA |
| Suggested primer 2: | GCGATACGAGGTGGGAGATA |