| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.130082 |
| Chromosome: | chromosome 1 |
| Location: | 6555031 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g046800 | MSP1 | Membrane selenoprotein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAATCTGTGAGCGCCGGCAGTAGCACGT |
| Internal bar code: | ACACGCTTCAACTGTCGCGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 772 |
| LEAP-Seq percent confirming: | 90.3536 |
| LEAP-Seq n confirming: | 2351 |
| LEAP-Seq n nonconfirming: | 251 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCTACTGGGTCAGCAAGG |
| Suggested primer 2: | CGACACCTTACGGGTGCTAT |