| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.130125 |
| Chromosome: | chromosome 7 |
| Location: | 3839684 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g338602 | POLH1 | (1 of 1) PTHR11076//PTHR11076:SF11 - DNA REPAIR POLYMERASE UMUC / TRANSFERASE FAMILY MEMBER // DNA POLYMERASE ETA; DNA polymerase eta subunit | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGAATGTAACGCCACCTCACCCCCCCCC |
| Internal bar code: | TCCAACGGTTCTTGATGTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 120 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 116 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACGCGCTATGAACTACGA |
| Suggested primer 2: | GCTAGTCGAACTGCCCTTTG |