| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.130173 |
| Chromosome: | chromosome 17 |
| Location: | 3561799 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g725500 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGTAGGCCGCGCCACTCGGAACCGAGCA |
| Internal bar code: | GGGCAGTTAGAGGGGTGCGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 747 |
| LEAP-Seq percent confirming: | 99.8866 |
| LEAP-Seq n confirming: | 1761 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCCGTCAATCCATGTTG |
| Suggested primer 2: | CGCCCTATCAAGACCAACAT |