| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.130186 |
| Chromosome: | chromosome_5 |
| Location: | 1494873 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g243801 | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTACGAGCGGCGTAGTCCCTGCTGAAAACC |
| Internal bar code: | GACGGGAGGCTCCGGCTCGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 736 |
| LEAP-Seq percent confirming: | 99.8752 |
| LEAP-Seq n confirming: | 1600 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGAAACTTGCGTGGATAAT |
| Suggested primer 2: | AGTTCCGATGACCGTTGAAG |