| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.130186 |
| Chromosome: | chromosome 5 |
| Location: | 1494875 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g243801 | FAP387 | Flagellar Associated Protein 387 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTACTGGTGAGAATGTAGCAGCAGGAAG |
| Internal bar code: | AGTTATTCTCGAATGTGGATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 469 |
| LEAP-Seq percent confirming: | 69.4196 |
| LEAP-Seq n confirming: | 311 |
| LEAP-Seq n nonconfirming: | 137 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGAAACTTGCGTGGATAAT |
| Suggested primer 2: | AGTTCCGATGACCGTTGAAG |