| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.130200 |
| Chromosome: | chromosome 6 |
| Location: | 3503439 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278105 | (1 of 24) IPR006311 - Twin-arginine translocation pathway, signal sequence | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGACGTGTCACACGCAAGCCGCAGCGTGGA |
| Internal bar code: | TCCGAGGAAGTGCGAGTGTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 904 |
| LEAP-Seq percent confirming: | 99.858 |
| LEAP-Seq n confirming: | 8439 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTCTGTCCCCATGTCCTC |
| Suggested primer 2: | AGTGCTGACTACCGGCCTTA |