| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.130272 |
| Chromosome: | chromosome 10 |
| Location: | 649074 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g422100 | (1 of 274) IPR020683 - Ankyrin repeat-containing domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGGAGCGAAGGTCAACACCACAGTCGTC |
| Internal bar code: | TGCGCGGCGGAACACCAGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 732 |
| LEAP-Seq percent confirming: | 99.8795 |
| LEAP-Seq n confirming: | 829 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCATACTCGTCATCCGTA |
| Suggested primer 2: | CACCTGTTAATGCGAGAGCA |