| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.130289 |
| Chromosome: | chromosome 15 |
| Location: | 783770 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g640000 | FAP345 | Flagellar Associated Protein 345 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAGTTCGGGGTGGGTTGTAAATACCAGC |
| Internal bar code: | GCTGCGTGTTGTTGGATTGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 325 |
| LEAP-Seq percent confirming: | 73.5088 |
| LEAP-Seq n confirming: | 419 |
| LEAP-Seq n nonconfirming: | 151 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGCGATTGTCAAAGCTGC |
| Suggested primer 2: | TTCTTAGGCATGTCCTTGGG |