Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.130289 |
Chromosome: | chromosome_15 |
Location: | 783770 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre15.g640000 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGGAGTTCGGGGTGGGTTGTAAATACCAGC |
Internal bar code: | GCTGCGTGTTGTTGGATTGACG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 325 |
LEAP-Seq percent confirming: | 73.5088 |
LEAP-Seq n confirming: | 419 |
LEAP-Seq n nonconfirming: | 151 |
LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TATGCGATTGTCAAAGCTGC |
Suggested primer 2: | TTCTTAGGCATGTCCTTGGG |