Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.130339 |
Chromosome: | chromosome 10 |
Location: | 3061191 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g441500 | (1 of 1) K09602 - ubiquitin thioesterase protein OTUB1 [EC:3.4.19.12] (OTUB1) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGTAGGAGTGGGCTGGGAGCCAGCAGGC |
Internal bar code: | GAGATCGGTTAAGAAACCGCGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 675 |
LEAP-Seq percent confirming: | 99.9421 |
LEAP-Seq n confirming: | 5175 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATAAAACGGGCGAAAGGAGT |
Suggested primer 2: | AGCTGTCTGCCACACATCAG |