| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.130369 |
| Chromosome: | chromosome 1 |
| Location: | 3511856 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g022500 | MME5 | NADP-dependent malic enzyme 5; (1 of 5) 1.1.1.40 - Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)) / Pyruvic-malic carboxylase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGCTGACGCCGGTTCCTGCCATCAACAG |
| Internal bar code: | CGCGGGATAGCTTGCCCAACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 777 |
| LEAP-Seq percent confirming: | 99.8924 |
| LEAP-Seq n confirming: | 1856 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAACTGCCTGGTGTTTCC |
| Suggested primer 2: | CAAGGCACGAGTGAACAAGA |