| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.130369 |
| Chromosome: | chromosome 1 |
| Location: | 6942518 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g050050 | GUM1 | Putative guanidinoacetate N-methyltransferase; (1 of 1) 2.1.1.2 - Guanidinoacetate N-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGACTTAGCCCTCGCCCGCCCCCCCCCTCC |
| Internal bar code: | TCCCAGTATATGTTGGGGTTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 631 |
| LEAP-Seq percent confirming: | 98.791 |
| LEAP-Seq n confirming: | 572 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGACCTTTTGTTAGGACA |
| Suggested primer 2: | CCGTACGACGGCATATTCTT |