| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.130381 |
| Chromosome: | chromosome 4 |
| Location: | 1956257 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g218450 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCACCGCCATCTTCCACTCGCCCTCGGGG |
| Internal bar code: | GCATTGATCAATGCTCATATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1016 |
| LEAP-Seq percent confirming: | 99.3952 |
| LEAP-Seq n confirming: | 19394 |
| LEAP-Seq n nonconfirming: | 118 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATCACAGCAAGCATAGGCG |
| Suggested primer 2: | GTAAATGCATGGGCTAGCGT |