| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.130383 |
| Chromosome: | chromosome 6 |
| Location: | 1300715 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g258363 | (1 of 1) IPR000048//IPR001611//IPR003591 - IQ motif, EF-hand binding site // Leucine-rich repeat // Leucine-rich repeat, typical subtype | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCGGCGCCGGCGACTACGGCGCCGCCGG |
| Internal bar code: | CAATGGGGTGTTGTTCTGAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 148 |
| LEAP-Seq percent confirming: | 96.9574 |
| LEAP-Seq n confirming: | 478 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCACGTGTCTCTGATAGCC |
| Suggested primer 2: | CCAGCTTCTTCCTGTTCTCG |