Insertion junction: LMJ.RY0402.130430_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.130430
Chromosome:chromosome 6
Location:7896518
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g303200 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCCGTCAACCTTCGTGGTGCTGGTCGTG
Internal bar code:TCCACAACCGCTGACTTCAGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:504
LEAP-Seq percent confirming:99.5826
LEAP-Seq n confirming:1193
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:AGGGGTCTTGATGGTGACAG
Suggested primer 2:AACACCTACACCGGCGTTAC