| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.130443 |
| Chromosome: | chromosome_12 |
| Location: | 7177440 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g558700 | FAP67 | Flagellar Associated Protein, nucleoside diphosphate kinase-like | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GAAAAATGGCAGCCAACCTGCGTTACTGCT |
| Internal bar code: | CCCCCCGGTAAAGTTGACTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 831 |
| LEAP-Seq percent confirming: | 99.6304 |
| LEAP-Seq n confirming: | 1887 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGATGGTGTTCCGGTGAT |
| Suggested primer 2: | TTCCCATATCTCCGAGCAAC |