| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.130488 |
| Chromosome: | chromosome 3 |
| Location: | 6404464 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g194300 | NDUFAF6 | (1 of 1) K18163 - NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 6 (NDUFAF6); NADH:ubiquinone oxidoreductase 1 alpha subcomplex assembly factor 6 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGAATTGTGCAGTGGATTGGCTCGGTTG |
| Internal bar code: | GAGAGGAGACAGTCATGGCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 153 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 1236 |
| LEAP-Seq n nonconfirming: | 309 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGCAGGGTTACAGTGGAC |
| Suggested primer 2: | CATGCTTTGAGCTGGCATAA |