| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.130495 |
| Chromosome: | chromosome 1 |
| Location: | 1957371 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g010550 | HLD2 | Haloalkane dehalogenase-like hydrolase; (1 of 2) 3.1.3.76 - Lipid-phosphate phosphatase / Soluble epoxide hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGCGAAGCGCGCGCTCTACCACTGAGCT |
| Internal bar code: | CGGGTCCTGATTAACAAGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 410 |
| LEAP-Seq percent confirming: | 90.8189 |
| LEAP-Seq n confirming: | 1098 |
| LEAP-Seq n nonconfirming: | 111 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCCTCTCACGTCACCAAT |
| Suggested primer 2: | TTGTGCTGCTAGGTTATGCG |