| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.130528 |
| Chromosome: | chromosome 15 |
| Location: | 1295765 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATAAGAGCGACCTAACCTCTGGCAAGCT |
| Internal bar code: | AATTCTCCCGGGAGCATAGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 803 |
| LEAP-Seq percent confirming: | 98.976 |
| LEAP-Seq n confirming: | 2223 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTGCTAGCCACGAGGTTT |
| Suggested primer 2: | TGCAGAACTGTAGGTCGTCG |