| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.130561 |
| Chromosome: | chromosome 10 |
| Location: | 645087 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g422050 | (1 of 1) PTHR22763:SF3 - E3 UBIQUITIN-PROTEIN LIGASE RNF115 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTATTTTTAGAATGGCCAGTCTGGCCAT |
| Internal bar code: | AAGCGGGAGCGAGGGCAGGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 483 |
| LEAP-Seq percent confirming: | 99.9265 |
| LEAP-Seq n confirming: | 1359 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTGGAGCGAAAGGATAGG |
| Suggested primer 2: | GGTACAGTGTTTGGGGCTGT |