| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.130563 |
| Chromosome: | chromosome 12 |
| Location: | 4285576 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g519550 | OPR53 | OctotricoPeptide Repeat protein 53 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGACGCAGAGGGATGTTACGAACCAGAGT |
| Internal bar code: | GCCATCGAGCGTAAAAAGCTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 167 |
| LEAP-Seq percent confirming: | 99.8674 |
| LEAP-Seq n confirming: | 753 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATCACGAGGTAGGCAAGGA |
| Suggested primer 2: | TTCTGATATCTTCCCACGCC |