| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.130576 |
| Chromosome: | chromosome 9 |
| Location: | 6789840 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g409500 | RPP25 | Ribonuclease MRP subunit P25; (1 of 2) PF01918 - Alba (Alba) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGAAGCTATGCTAAAATGCGTATCGGAGA |
| Internal bar code: | GGCCCTGCACGTCAAGCAGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 973 |
| LEAP-Seq percent confirming: | 99.8374 |
| LEAP-Seq n confirming: | 3071 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGGACAGTGGTAAGGCAG |
| Suggested primer 2: | TAGTCGTAGGAGGGGTGTGG |