| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.130596 |
| Chromosome: | chromosome 2 |
| Location: | 7603621 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g143600 | FEE1 | (1 of 1) PTHR12436:SF3 - GERMINAL-CENTER ASSOCIATED NUCLEAR PROTEIN; Feruloyl esterase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGTGTACTCATGTGTATGTGTGTTGTGA |
| Internal bar code: | AGTCTTGTGAACATCGCGAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 259 |
| LEAP-Seq percent confirming: | 98.923 |
| LEAP-Seq n confirming: | 3582 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTACCCCATTTGCTTGAT |
| Suggested primer 2: | ATATCGTGGAATAGCGCGAC |