| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.130602 |
| Chromosome: | chromosome 1 |
| Location: | 3590237 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g023150 | MCM5 | (1 of 1) K02209 - DNA replication licensing factor MCM5 (MCM5, CDC46); Minichromosome maintenance protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTCACAGCACACCAAGCAATCCCCCTCT |
| Internal bar code: | GCAGCAGGGTTGGCGGTGATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 115 |
| LEAP-Seq percent confirming: | 99.8656 |
| LEAP-Seq n confirming: | 4457 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGCTGTTCACAAAGTCCA |
| Suggested primer 2: | CGGGATAAGAAACGACGAAA |