| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.130607 |
| Chromosome: | chromosome 7 |
| Location: | 2343479 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g328150 | PDI4 | Protein disulfide isomerase 4; (1 of 1) PTHR18929:SF38 - PROTEIN DISULFIDE-ISOMERASE A6 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGACAACCCCAGCGCCCCCCCCCTCTCT |
| Internal bar code: | ACCATGTTTAGAGAGCCAATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 313 |
| LEAP-Seq percent confirming: | 88.2001 |
| LEAP-Seq n confirming: | 1181 |
| LEAP-Seq n nonconfirming: | 158 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTGTCCGAACCAACTTGT |
| Suggested primer 2: | AGCGTCTTGATGTAGCGGTT |