Insertion junction: LMJ.RY0402.130613_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.130613
Chromosome:chromosome 2
Location:1996333
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g088350 SNR3 (1 of 6) PF09335 - SNARE associated Golgi protein (SNARE_assoc); SNARE associated Golgi protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCACCAGCCTTCGCAGCTCTGAACAACAG
Internal bar code:CGCAGTGGAGCGTGTTGAGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:876
LEAP-Seq percent confirming:99.912
LEAP-Seq n confirming:10217
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:59

Suggested primers for confirmation by PCR

Suggested primer 1:CCAGCACAGCCTTTTTCTTC
Suggested primer 2:CAGTAGGGTGCTGTGGGAAT