| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.130614 |
| Chromosome: | chromosome 3 |
| Location: | 4065549 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g172600 | (1 of 1) IPR000104//IPR001841//IPR011990//IPR013083 - Antifreeze protein, type I // Zinc finger, RING-type // Tetratricopeptide-like helical domain // Zinc finger, RING/FYVE/PHD-type | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACGAACTGCACCTCTTTGTATGGCTGT |
| Internal bar code: | GGCGGTGATGAGATAGGAGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1175 |
| LEAP-Seq percent confirming: | 99.7883 |
| LEAP-Seq n confirming: | 17440 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAATCATCACACCAAGGGCA |
| Suggested primer 2: | AGGTGGCGGATGTCAATATG |