Insertion junction: LMJ.RY0402.130620_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.130620
Chromosome:chromosome 7
Location:5926303
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g354150 MFT22,ARSJ1 Major facilitator superfamily transporter; (1 of 82) IPR020846 - Major facilitator superfamily domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCACACACACATGACGCCCTCAGCCCGC
Internal bar code:TATAGGGCATAACCACGTGCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:760
LEAP-Seq percent confirming:99.7871
LEAP-Seq n confirming:4686
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:GCGCAGTAGTAGAAGCCCAC
Suggested primer 2:GGCTTTGTGTGTTGGAGGTT