| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.130633 |
| Chromosome: | chromosome 1 |
| Location: | 2958399 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g018075 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTTTGCAAGCTGCCACTGAGCAAAGAAC |
| Internal bar code: | GGGCACTATAGTCTTCGTGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 679 |
| LEAP-Seq percent confirming: | 99.036 |
| LEAP-Seq n confirming: | 4623 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGAGCAGATGACGGTGTA |
| Suggested primer 2: | TGTACTCTATCGCCAGCACG |