Insertion junction: LMJ.RY0402.130640_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.130640
Chromosome:chromosome 16
Location:2076545
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g657450 MPA12 Metallophosphoesterase/metallo-dependent phosphatase; (1 of 1) 3.6.1.13//3.6.1.16//3.6.1.53 - ADP-ribose diphosphatase / ADPR-PPase // CDP-glycerol diphosphatase / CDP-glycerol pyrophosphatase // Mn(2+)-dependent ADP-ribose/CDP-alcohol diphosphatase / Mn(2+)-dependent ADP-ribose/CDP-alcohol pyrophosphatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAGTTGTATGGCGCTGTACGGCAGCGCT
Internal bar code:TCACGCGCTAGGATGGGGCCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:501
LEAP-Seq percent confirming:98.8265
LEAP-Seq n confirming:2779
LEAP-Seq n nonconfirming:33
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:TGCAGGGTTAGAGGAGGCTA
Suggested primer 2:GCGGGTAGATAATGCGTGTT