Insertion junction: LMJ.RY0402.130676_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.130676
Chromosome:chromosome 17
Location:252209
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g697950 AAA3 Plastidic ADP/ATP translocase; (1 of 2) PTHR31187:SF1 - ADP,ATP CARRIER PROTEIN 1, CHLOROPLASTIC-RELATED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGACACCCCTTGCGGACGACCACCGACCGC
Internal bar code:AAGCGGCTCGGAAGAGTGTTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:155
LEAP-Seq percent confirming:74.3088
LEAP-Seq n confirming:1290
LEAP-Seq n nonconfirming:446
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CGGGTACAACAGGAAAGGAA
Suggested primer 2:GGTTTTGGGACTGCACTGTT