| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.130679 |
| Chromosome: | chromosome 16 |
| Location: | 5617048 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g679750 | (1 of 2) PTHR17630//PTHR17630:SF43 - DIENELACTONE HYDROLASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCATGATGCCGATGGCCTTGCCGGCGGCC |
| Internal bar code: | CGAGCCTGGGGCCCAGGGTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1015 |
| LEAP-Seq percent confirming: | 98.8581 |
| LEAP-Seq n confirming: | 4069 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTGCCAGGGAAAGTTAT |
| Suggested primer 2: | CACATGTCACTATCGCCACC |