| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.130698 |
| Chromosome: | chromosome 15 |
| Location: | 429117 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g636300 | CPN2 | Tubulin folding cofactor B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGCACCACCAGGGGGCACCACCAAGAG |
| Internal bar code: | GTAACCCGGTCAAGAGTCATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 476 |
| LEAP-Seq percent confirming: | 99.8811 |
| LEAP-Seq n confirming: | 2520 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGCTTTGACCGACACTGA |
| Suggested primer 2: | AATGGGAGGCAGGACTAGGT |