| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.130706 |
| Chromosome: | chromosome 17 |
| Location: | 3216119 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g722300 | (1 of 26) IPR017850 - Alkaline-phosphatase-like, core domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGGCATCTACTCTGGCTACGCCTATGGC |
| Internal bar code: | GGACCGCTGGGTCCCAGAAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 827 |
| LEAP-Seq percent confirming: | 99.724 |
| LEAP-Seq n confirming: | 3974 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGGTGCTGATGTGGAGAT |
| Suggested primer 2: | GCTTACACGTGCTCACTCCA |