| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.130713 |
| Chromosome: | chromosome 7 |
| Location: | 3582969 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g336650 | MLH3 | DNA mismatch repair protein; (1 of 1) IPR003594//IPR020568 - Histidine kinase-like ATPase, C-terminal domain // Ribosomal protein S5 domain 2-type fold | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAGTGCAGGGCCGGCTCTAGCTACTGAC |
| Internal bar code: | TCGGGACTCGAACCGAAGGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 867 |
| LEAP-Seq percent confirming: | 99.1926 |
| LEAP-Seq n confirming: | 3317 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAGGCTTAAGCACGCAGGT |
| Suggested primer 2: | CTTATCGACTAGCGGCTTGG |