| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.130727 |
| Chromosome: | chromosome 6 |
| Location: | 8208917 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g305516 | 5'UTR_intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTACCAGCACCCGTCGCCATCCCCCAATG |
| Internal bar code: | AGGGGAGAGACGGTTTCTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 910 |
| LEAP-Seq percent confirming: | 99.4077 |
| LEAP-Seq n confirming: | 5371 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGGCAAACAATGTGCAAC |
| Suggested primer 2: | TGCAGTTTGCCTCACTTGTC |