| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.130732 |
| Chromosome: | chromosome_13 |
| Location: | 2071710 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g577352 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTGTTTTGGCCCGCAACCACCGTCACATCC |
| Internal bar code: | GTAGGTCGGGGATCACACGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 906 |
| LEAP-Seq percent confirming: | 99.7284 |
| LEAP-Seq n confirming: | 5141 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCATCCTGCCCTTAGGAGT |
| Suggested primer 2: | CTCGCTGAGAACTGACCACA |