| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.130748 |
| Chromosome: | chromosome_5 |
| Location: | 1378941 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g244350 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GAGCCGCCCTCACAAAATTGTAACACGACG |
| Internal bar code: | CCGGACTGCACGCTTGCCTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 525 |
| LEAP-Seq percent confirming: | 89.4423 |
| LEAP-Seq n confirming: | 1652 |
| LEAP-Seq n nonconfirming: | 195 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTGTCCGGCATACCACTT |
| Suggested primer 2: | GTGGGCTATCTGCTAGGCTG |