Insertion junction: LMJ.RY0402.130748_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.130748
Chromosome:chromosome 9
Location:3677403
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g390763 RAB2,FAP354 Rab-like GTP-Binding Flagellar Associated Protein 354; (1 of 1) K07877 - Ras-related protein Rab-2A (RAB2A) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAATGCACTCACACACACACACAGACACA
Internal bar code:GCCCAGGGCCAGACGCGGGGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:827
LEAP-Seq percent confirming:97.4858
LEAP-Seq n confirming:2404
LEAP-Seq n nonconfirming:62
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:ATATTTGCCTCACTCACCCG
Suggested primer 2:CGCTTGTGTGAGCTTTGGTA