Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.130768 |
Chromosome: | chromosome_2 |
Location: | 7235594 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g145850 | FAP112 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGGGACACCCTGGCCCGGGCCTCAGGTTTC |
Internal bar code: | GACATCCCTGCGGGGGGGGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 809 |
LEAP-Seq percent confirming: | 99.4393 |
LEAP-Seq n confirming: | 532 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CACTGCCATCACATACCTGG |
Suggested primer 2: | GGGTCGTGAAAATCATTGCT |