| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.130787 |
| Chromosome: | chromosome 9 |
| Location: | 6151204 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g405600 | SFT1 | (1 of 1) K08505 - protein transport protein SFT1 (SFT1); Intra-Golgi Qc-SNARE protein, Gs15/Sft1 family (Qc.II) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGGGCGAGGACGTGTGCGTGTCACGCAA |
| Internal bar code: | ATGTGGTAGGAGCTCGGTGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 359 |
| LEAP-Seq percent confirming: | 83.9806 |
| LEAP-Seq n confirming: | 173 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACCACATGCTGTACCTGG |
| Suggested primer 2: | TGCTGTTCCTCGTTTCAGTG |